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anti-map2 (goat)  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology anti-map2 (goat)

    Anti Map2 (Goat), supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+map2+antibody/map2+antibody/pmc11335265-10-0-3
    Average 90 stars, based on 1 article reviews
    anti-map2 (goat) - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Non-canonical function of ADAM10 in presynaptic plasticity"

    Article Title: Non-canonical function of ADAM10 in presynaptic plasticity

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-024-05327-8


    Figure Legend Snippet:

    Techniques Used: Plasmid Preparation, Recombinant, Blocking Assay, Control, In Situ, Knock-Out, Software, Microscopy

    Related Articles

    Expressing:

    Article Title: Translocator protein (18 kDa) (TSPO) is expressed in reactive retinal microglia and modulates microglial inflammation and phagocytosis
    Article Snippet: Sections were blocked with a dried milk solution followed by an overnight incubation with primary antibodies at 4°C.Sections were blocked with a dried milk solution followed by an overnight incubation with primary antibodies at 4°C.. Antibodies included rabbit anti-Iba1 antibody (Wako Chemicals, Neuss, Germany), rabbit anti-TSPO antibody (Abcam, Cambridge, UK), goat anti-MAP2 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and goat anti-GFAP antibody (Santa Cruz Biotechnology).. After washing, samples were labeled with a secondary antibody conjugated to Alexa488 (green) or Alexa594 (red) (Jackson Immuno-Research, West Grove, PA, USA) and counter-stained with DAPI.After washing, samples were labeled with a secondary antibody conjugated to Alexa488 (green) or Alexa594 (red) (Jackson Immuno-Research, West Grove, PA, USA) and counter-stained with DAPI.

    Article Title: Mifepristone-inducible caspase-1 expression in mouse embryonic stem cells eliminates tumor formation but spares differentiated cells in vitro and in vivo.
    Article Snippet: After fixing the cells and sections, primary antibodies were applied as follows: mouse anti–microtubule-associated protein 2 (anti-MAP2) antibody (1:2,000, M4403, Sigma), rabbit anti-tyrosine hydroxylase (TH) antibody (1:500, AB152, Chemicon), mouse anti–sex-determining region Y protein (anti-SRY) antibody (1:200, ab22166, Abcam, UK, www.abcam.com), rabbit anti-Oct-4 antibody (1:200, 2750, Cell Signaling), mouse anti-nestin antibody (1:200, MAB353, Chemicon), and goat anti-MAP2 antibody (1:20, sc-5359, Santa Cruz).. The second antibodies were applied as follows: Cy3-conjugated goat antimouse IgG (1:500, 115-165-062, Jackson ImmunoResearch, Bar Harbor, ME, www.jacksonimmuno.com), Cy2-conjugated goat anti-goat IgG (1:500, 111-225-045, Jackson ImmunoResearch), Alexa Fluor 350 donkey anti-goat IgG (1:200, A21081, Invitrogen).The second antibodies were applied as follows: Cy3-conjugated goat antimouse IgG (1:500, 115-165-062, Jackson ImmunoResearch, Bar Harbor, ME, www.jacksonimmuno.com), Cy2-conjugated goat anti-goat IgG (1:500, 111-225-045, Jackson ImmunoResearch), Alexa Fluor 350..

    Marker:

    Article Title: Translocator protein (18 kDa) (TSPO) is expressed in reactive retinal microglia and modulates microglial inflammation and phagocytosis
    Article Snippet: Sections were blocked with a dried milk solution followed by an overnight incubation with primary antibodies at 4°C.Sections were blocked with a dried milk solution followed by an overnight incubation with primary antibodies at 4°C.. Antibodies included rabbit anti-Iba1 antibody (Wako Chemicals, Neuss, Germany), rabbit anti-TSPO antibody (Abcam, Cambridge, UK), goat anti-MAP2 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and goat anti-GFAP antibody (Santa Cruz Biotechnology).. After washing, samples were labeled with a secondary antibody conjugated to Alexa488 (green) or Alexa594 (red) (Jackson Immuno-Research, West Grove, PA, USA) and counter-stained with DAPI.After washing, samples were labeled with a secondary antibody conjugated to Alexa488 (green) or Alexa594 (red) (Jackson Immuno-Research, West Grove, PA, USA) and counter-stained with DAPI.

    Article Title: Mifepristone-inducible caspase-1 expression in mouse embryonic stem cells eliminates tumor formation but spares differentiated cells in vitro and in vivo.
    Article Snippet: After fixing the cells and sections, primary antibodies were applied as follows: mouse anti–microtubule-associated protein 2 (anti-MAP2) antibody (1:2,000, M4403, Sigma), rabbit anti-tyrosine hydroxylase (TH) antibody (1:500, AB152, Chemicon), mouse anti–sex-determining region Y protein (anti-SRY) antibody (1:200, ab22166, Abcam, UK, www.abcam.com), rabbit anti-Oct-4 antibody (1:200, 2750, Cell Signaling), mouse anti-nestin antibody (1:200, MAB353, Chemicon), and goat anti-MAP2 antibody (1:20, sc-5359, Santa Cruz).. The second antibodies were applied as follows: Cy3-conjugated goat antimouse IgG (1:500, 115-165-062, Jackson ImmunoResearch, Bar Harbor, ME, www.jacksonimmuno.com), Cy2-conjugated goat anti-goat IgG (1:500, 111-225-045, Jackson ImmunoResearch), Alexa Fluor 350 donkey anti-goat IgG (1:200, A21081, Invitrogen).The second antibodies were applied as follows: Cy3-conjugated goat antimouse IgG (1:500, 115-165-062, Jackson ImmunoResearch, Bar Harbor, ME, www.jacksonimmuno.com), Cy2-conjugated goat anti-goat IgG (1:500, 111-225-045, Jackson ImmunoResearch), Alexa Fluor 350..

    Immunofluorescence:

    Article Title: Translocator protein (18 kDa) (TSPO) is expressed in reactive retinal microglia and modulates microglial inflammation and phagocytosis
    Article Snippet: Sections were blocked with a dried milk solution followed by an overnight incubation with primary antibodies at 4°C.Sections were blocked with a dried milk solution followed by an overnight incubation with primary antibodies at 4°C.. Antibodies included rabbit anti-Iba1 antibody (Wako Chemicals, Neuss, Germany), rabbit anti-TSPO antibody (Abcam, Cambridge, UK), goat anti-MAP2 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and goat anti-GFAP antibody (Santa Cruz Biotechnology).. After washing, samples were labeled with a secondary antibody conjugated to Alexa488 (green) or Alexa594 (red) (Jackson Immuno-Research, West Grove, PA, USA) and counter-stained with DAPI.After washing, samples were labeled with a secondary antibody conjugated to Alexa488 (green) or Alexa594 (red) (Jackson Immuno-Research, West Grove, PA, USA) and counter-stained with DAPI.

    Article Title: Mifepristone-inducible caspase-1 expression in mouse embryonic stem cells eliminates tumor formation but spares differentiated cells in vitro and in vivo.
    Article Snippet: After fixing the cells and sections, primary antibodies were applied as follows: mouse anti–microtubule-associated protein 2 (anti-MAP2) antibody (1:2,000, M4403, Sigma), rabbit anti-tyrosine hydroxylase (TH) antibody (1:500, AB152, Chemicon), mouse anti–sex-determining region Y protein (anti-SRY) antibody (1:200, ab22166, Abcam, UK, www.abcam.com), rabbit anti-Oct-4 antibody (1:200, 2750, Cell Signaling), mouse anti-nestin antibody (1:200, MAB353, Chemicon), and goat anti-MAP2 antibody (1:20, sc-5359, Santa Cruz).. The second antibodies were applied as follows: Cy3-conjugated goat antimouse IgG (1:500, 115-165-062, Jackson ImmunoResearch, Bar Harbor, ME, www.jacksonimmuno.com), Cy2-conjugated goat anti-goat IgG (1:500, 111-225-045, Jackson ImmunoResearch), Alexa Fluor 350 donkey anti-goat IgG (1:200, A21081, Invitrogen).The second antibodies were applied as follows: Cy3-conjugated goat antimouse IgG (1:500, 115-165-062, Jackson ImmunoResearch, Bar Harbor, ME, www.jacksonimmuno.com), Cy2-conjugated goat anti-goat IgG (1:500, 111-225-045, Jackson ImmunoResearch), Alexa Fluor 350..

    Immunostaining:

    Article Title: Translocator protein (18 kDa) (TSPO) is expressed in reactive retinal microglia and modulates microglial inflammation and phagocytosis
    Article Snippet: Sections were blocked with a dried milk solution followed by an overnight incubation with primary antibodies at 4°C.Sections were blocked with a dried milk solution followed by an overnight incubation with primary antibodies at 4°C.. Antibodies included rabbit anti-Iba1 antibody (Wako Chemicals, Neuss, Germany), rabbit anti-TSPO antibody (Abcam, Cambridge, UK), goat anti-MAP2 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and goat anti-GFAP antibody (Santa Cruz Biotechnology).. After washing, samples were labeled with a secondary antibody conjugated to Alexa488 (green) or Alexa594 (red) (Jackson Immuno-Research, West Grove, PA, USA) and counter-stained with DAPI.After washing, samples were labeled with a secondary antibody conjugated to Alexa488 (green) or Alexa594 (red) (Jackson Immuno-Research, West Grove, PA, USA) and counter-stained with DAPI.

    Article Title: Mifepristone-inducible caspase-1 expression in mouse embryonic stem cells eliminates tumor formation but spares differentiated cells in vitro and in vivo.
    Article Snippet: After fixing the cells and sections, primary antibodies were applied as follows: mouse anti–microtubule-associated protein 2 (anti-MAP2) antibody (1:2,000, M4403, Sigma), rabbit anti-tyrosine hydroxylase (TH) antibody (1:500, AB152, Chemicon), mouse anti–sex-determining region Y protein (anti-SRY) antibody (1:200, ab22166, Abcam, UK, www.abcam.com), rabbit anti-Oct-4 antibody (1:200, 2750, Cell Signaling), mouse anti-nestin antibody (1:200, MAB353, Chemicon), and goat anti-MAP2 antibody (1:20, sc-5359, Santa Cruz).. The second antibodies were applied as follows: Cy3-conjugated goat antimouse IgG (1:500, 115-165-062, Jackson ImmunoResearch, Bar Harbor, ME, www.jacksonimmuno.com), Cy2-conjugated goat anti-goat IgG (1:500, 111-225-045, Jackson ImmunoResearch), Alexa Fluor 350 donkey anti-goat IgG (1:200, A21081, Invitrogen).The second antibodies were applied as follows: Cy3-conjugated goat antimouse IgG (1:500, 115-165-062, Jackson ImmunoResearch, Bar Harbor, ME, www.jacksonimmuno.com), Cy2-conjugated goat anti-goat IgG (1:500, 111-225-045, Jackson ImmunoResearch), Alexa Fluor 350..

    Staining:

    Article Title: Translocator protein (18 kDa) (TSPO) is expressed in reactive retinal microglia and modulates microglial inflammation and phagocytosis
    Article Snippet: Sections were blocked with a dried milk solution followed by an overnight incubation with primary antibodies at 4°C.Sections were blocked with a dried milk solution followed by an overnight incubation with primary antibodies at 4°C.. Antibodies included rabbit anti-Iba1 antibody (Wako Chemicals, Neuss, Germany), rabbit anti-TSPO antibody (Abcam, Cambridge, UK), goat anti-MAP2 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and goat anti-GFAP antibody (Santa Cruz Biotechnology).. After washing, samples were labeled with a secondary antibody conjugated to Alexa488 (green) or Alexa594 (red) (Jackson Immuno-Research, West Grove, PA, USA) and counter-stained with DAPI.After washing, samples were labeled with a secondary antibody conjugated to Alexa488 (green) or Alexa594 (red) (Jackson Immuno-Research, West Grove, PA, USA) and counter-stained with DAPI.

    Article Title: Mifepristone-inducible caspase-1 expression in mouse embryonic stem cells eliminates tumor formation but spares differentiated cells in vitro and in vivo.
    Article Snippet: After fixing the cells and sections, primary antibodies were applied as follows: mouse anti–microtubule-associated protein 2 (anti-MAP2) antibody (1:2,000, M4403, Sigma), rabbit anti-tyrosine hydroxylase (TH) antibody (1:500, AB152, Chemicon), mouse anti–sex-determining region Y protein (anti-SRY) antibody (1:200, ab22166, Abcam, UK, www.abcam.com), rabbit anti-Oct-4 antibody (1:200, 2750, Cell Signaling), mouse anti-nestin antibody (1:200, MAB353, Chemicon), and goat anti-MAP2 antibody (1:20, sc-5359, Santa Cruz).. The second antibodies were applied as follows: Cy3-conjugated goat antimouse IgG (1:500, 115-165-062, Jackson ImmunoResearch, Bar Harbor, ME, www.jacksonimmuno.com), Cy2-conjugated goat anti-goat IgG (1:500, 111-225-045, Jackson ImmunoResearch), Alexa Fluor 350 donkey anti-goat IgG (1:200, A21081, Invitrogen).The second antibodies were applied as follows: Cy3-conjugated goat antimouse IgG (1:500, 115-165-062, Jackson ImmunoResearch, Bar Harbor, ME, www.jacksonimmuno.com), Cy2-conjugated goat anti-goat IgG (1:500, 111-225-045, Jackson ImmunoResearch), Alexa Fluor 350..

    Derivative Assay:

    Article Title: Translocator protein (18 kDa) (TSPO) is expressed in reactive retinal microglia and modulates microglial inflammation and phagocytosis
    Article Snippet: Sections were blocked with a dried milk solution followed by an overnight incubation with primary antibodies at 4°C.Sections were blocked with a dried milk solution followed by an overnight incubation with primary antibodies at 4°C.. Antibodies included rabbit anti-Iba1 antibody (Wako Chemicals, Neuss, Germany), rabbit anti-TSPO antibody (Abcam, Cambridge, UK), goat anti-MAP2 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and goat anti-GFAP antibody (Santa Cruz Biotechnology).. After washing, samples were labeled with a secondary antibody conjugated to Alexa488 (green) or Alexa594 (red) (Jackson Immuno-Research, West Grove, PA, USA) and counter-stained with DAPI.After washing, samples were labeled with a secondary antibody conjugated to Alexa488 (green) or Alexa594 (red) (Jackson Immuno-Research, West Grove, PA, USA) and counter-stained with DAPI.

    Article Title: Mifepristone-inducible caspase-1 expression in mouse embryonic stem cells eliminates tumor formation but spares differentiated cells in vitro and in vivo.
    Article Snippet: After fixing the cells and sections, primary antibodies were applied as follows: mouse anti–microtubule-associated protein 2 (anti-MAP2) antibody (1:2,000, M4403, Sigma), rabbit anti-tyrosine hydroxylase (TH) antibody (1:500, AB152, Chemicon), mouse anti–sex-determining region Y protein (anti-SRY) antibody (1:200, ab22166, Abcam, UK, www.abcam.com), rabbit anti-Oct-4 antibody (1:200, 2750, Cell Signaling), mouse anti-nestin antibody (1:200, MAB353, Chemicon), and goat anti-MAP2 antibody (1:20, sc-5359, Santa Cruz).. The second antibodies were applied as follows: Cy3-conjugated goat antimouse IgG (1:500, 115-165-062, Jackson ImmunoResearch, Bar Harbor, ME, www.jacksonimmuno.com), Cy2-conjugated goat anti-goat IgG (1:500, 111-225-045, Jackson ImmunoResearch), Alexa Fluor 350 donkey anti-goat IgG (1:200, A21081, Invitrogen).The second antibodies were applied as follows: Cy3-conjugated goat antimouse IgG (1:500, 115-165-062, Jackson ImmunoResearch, Bar Harbor, ME, www.jacksonimmuno.com), Cy2-conjugated goat anti-goat IgG (1:500, 111-225-045, Jackson ImmunoResearch), Alexa Fluor 350..

    In Vitro:

    Article Title: Translocator protein (18 kDa) (TSPO) is expressed in reactive retinal microglia and modulates microglial inflammation and phagocytosis
    Article Snippet: Sections were blocked with a dried milk solution followed by an overnight incubation with primary antibodies at 4°C.Sections were blocked with a dried milk solution followed by an overnight incubation with primary antibodies at 4°C.. Antibodies included rabbit anti-Iba1 antibody (Wako Chemicals, Neuss, Germany), rabbit anti-TSPO antibody (Abcam, Cambridge, UK), goat anti-MAP2 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and goat anti-GFAP antibody (Santa Cruz Biotechnology).. After washing, samples were labeled with a secondary antibody conjugated to Alexa488 (green) or Alexa594 (red) (Jackson Immuno-Research, West Grove, PA, USA) and counter-stained with DAPI.After washing, samples were labeled with a secondary antibody conjugated to Alexa488 (green) or Alexa594 (red) (Jackson Immuno-Research, West Grove, PA, USA) and counter-stained with DAPI.

    Article Title: Mifepristone-inducible caspase-1 expression in mouse embryonic stem cells eliminates tumor formation but spares differentiated cells in vitro and in vivo.
    Article Snippet: After fixing the cells and sections, primary antibodies were applied as follows: mouse anti–microtubule-associated protein 2 (anti-MAP2) antibody (1:2,000, M4403, Sigma), rabbit anti-tyrosine hydroxylase (TH) antibody (1:500, AB152, Chemicon), mouse anti–sex-determining region Y protein (anti-SRY) antibody (1:200, ab22166, Abcam, UK, www.abcam.com), rabbit anti-Oct-4 antibody (1:200, 2750, Cell Signaling), mouse anti-nestin antibody (1:200, MAB353, Chemicon), and goat anti-MAP2 antibody (1:20, sc-5359, Santa Cruz).. The second antibodies were applied as follows: Cy3-conjugated goat antimouse IgG (1:500, 115-165-062, Jackson ImmunoResearch, Bar Harbor, ME, www.jacksonimmuno.com), Cy2-conjugated goat anti-goat IgG (1:500, 111-225-045, Jackson ImmunoResearch), Alexa Fluor 350 donkey anti-goat IgG (1:200, A21081, Invitrogen).The second antibodies were applied as follows: Cy3-conjugated goat antimouse IgG (1:500, 115-165-062, Jackson ImmunoResearch, Bar Harbor, ME, www.jacksonimmuno.com), Cy2-conjugated goat anti-goat IgG (1:500, 111-225-045, Jackson ImmunoResearch), Alexa Fluor 350..

    Fluorescence:

    Article Title: Translocator protein (18 kDa) (TSPO) is expressed in reactive retinal microglia and modulates microglial inflammation and phagocytosis
    Article Snippet: Sections were blocked with a dried milk solution followed by an overnight incubation with primary antibodies at 4°C.Sections were blocked with a dried milk solution followed by an overnight incubation with primary antibodies at 4°C.. Antibodies included rabbit anti-Iba1 antibody (Wako Chemicals, Neuss, Germany), rabbit anti-TSPO antibody (Abcam, Cambridge, UK), goat anti-MAP2 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and goat anti-GFAP antibody (Santa Cruz Biotechnology).. After washing, samples were labeled with a secondary antibody conjugated to Alexa488 (green) or Alexa594 (red) (Jackson Immuno-Research, West Grove, PA, USA) and counter-stained with DAPI.After washing, samples were labeled with a secondary antibody conjugated to Alexa488 (green) or Alexa594 (red) (Jackson Immuno-Research, West Grove, PA, USA) and counter-stained with DAPI.

    Article Title: Mifepristone-inducible caspase-1 expression in mouse embryonic stem cells eliminates tumor formation but spares differentiated cells in vitro and in vivo.
    Article Snippet: After fixing the cells and sections, primary antibodies were applied as follows: mouse anti–microtubule-associated protein 2 (anti-MAP2) antibody (1:2,000, M4403, Sigma), rabbit anti-tyrosine hydroxylase (TH) antibody (1:500, AB152, Chemicon), mouse anti–sex-determining region Y protein (anti-SRY) antibody (1:200, ab22166, Abcam, UK, www.abcam.com), rabbit anti-Oct-4 antibody (1:200, 2750, Cell Signaling), mouse anti-nestin antibody (1:200, MAB353, Chemicon), and goat anti-MAP2 antibody (1:20, sc-5359, Santa Cruz).. The second antibodies were applied as follows: Cy3-conjugated goat antimouse IgG (1:500, 115-165-062, Jackson ImmunoResearch, Bar Harbor, ME, www.jacksonimmuno.com), Cy2-conjugated goat anti-goat IgG (1:500, 111-225-045, Jackson ImmunoResearch), Alexa Fluor 350 donkey anti-goat IgG (1:200, A21081, Invitrogen).The second antibodies were applied as follows: Cy3-conjugated goat antimouse IgG (1:500, 115-165-062, Jackson ImmunoResearch, Bar Harbor, ME, www.jacksonimmuno.com), Cy2-conjugated goat anti-goat IgG (1:500, 111-225-045, Jackson ImmunoResearch), Alexa Fluor 350..

    FACS:

    Article Title: Translocator protein (18 kDa) (TSPO) is expressed in reactive retinal microglia and modulates microglial inflammation and phagocytosis
    Article Snippet: Sections were blocked with a dried milk solution followed by an overnight incubation with primary antibodies at 4°C.Sections were blocked with a dried milk solution followed by an overnight incubation with primary antibodies at 4°C.. Antibodies included rabbit anti-Iba1 antibody (Wako Chemicals, Neuss, Germany), rabbit anti-TSPO antibody (Abcam, Cambridge, UK), goat anti-MAP2 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and goat anti-GFAP antibody (Santa Cruz Biotechnology).. After washing, samples were labeled with a secondary antibody conjugated to Alexa488 (green) or Alexa594 (red) (Jackson Immuno-Research, West Grove, PA, USA) and counter-stained with DAPI.After washing, samples were labeled with a secondary antibody conjugated to Alexa488 (green) or Alexa594 (red) (Jackson Immuno-Research, West Grove, PA, USA) and counter-stained with DAPI.

    Article Title: Mifepristone-inducible caspase-1 expression in mouse embryonic stem cells eliminates tumor formation but spares differentiated cells in vitro and in vivo.
    Article Snippet: After fixing the cells and sections, primary antibodies were applied as follows: mouse anti–microtubule-associated protein 2 (anti-MAP2) antibody (1:2,000, M4403, Sigma), rabbit anti-tyrosine hydroxylase (TH) antibody (1:500, AB152, Chemicon), mouse anti–sex-determining region Y protein (anti-SRY) antibody (1:200, ab22166, Abcam, UK, www.abcam.com), rabbit anti-Oct-4 antibody (1:200, 2750, Cell Signaling), mouse anti-nestin antibody (1:200, MAB353, Chemicon), and goat anti-MAP2 antibody (1:20, sc-5359, Santa Cruz).. The second antibodies were applied as follows: Cy3-conjugated goat antimouse IgG (1:500, 115-165-062, Jackson ImmunoResearch, Bar Harbor, ME, www.jacksonimmuno.com), Cy2-conjugated goat anti-goat IgG (1:500, 111-225-045, Jackson ImmunoResearch), Alexa Fluor 350 donkey anti-goat IgG (1:200, A21081, Invitrogen).The second antibodies were applied as follows: Cy3-conjugated goat antimouse IgG (1:500, 115-165-062, Jackson ImmunoResearch, Bar Harbor, ME, www.jacksonimmuno.com), Cy2-conjugated goat anti-goat IgG (1:500, 111-225-045, Jackson ImmunoResearch), Alexa Fluor 350..



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    PhosphoSolutions anti map2 goat polyclonal igg antibody
    Fig. 3 | Role of the UPR and Wnt/β-catenin signaling in nuclear REST induction. a Immunolabeling of UPR activation (marker BiP/GRP78, red), REST (green), β- catenin (magenta) and DNA (DAPI, blue) in 11-month-old 3xTg and WT mice shows coordinate upregulation of BiP, nuclear REST and nuclear β-catenin expression in 3xTg mice. b Correlation between nuclear β-catenin and nuclear REST (left graph), BiP and nuclear REST (middle graph) and BiP and nuclear β-catenin (right graph) levels in the hippocampus CA1 region of 11-month-old 3xTg mice. Shown are the mean fluorescence intensity values for nuclear REST in individual CA1 neurons from n = 3 3xTg mice. a.u.- arbitrary units. The Pearson correlation coefficient (r) and P- value are shown. c Immunolabeling of REST (green) and neuron marker <t>MAP2</t> (red) in 3xTg primary cortical neurons (PCNs) shows nuclear REST in neurons treated with vehicle and decreased nuclear REST in 3xTg neurons after a 24-h treatment with the Wnt/β-catenin inhibitors Dickkopf 1 (DKK1), XAV939 and ICG001, or the PERK inhibitor GSK2606414. d Quantification of average nuclear REST levels in WT PCNs, as well as 3xTg PCNs treated with vehicle or the individual drugs. The P-
    Anti Map2 Goat Polyclonal Igg Antibody, supplied by PhosphoSolutions, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti map2  (Danaher Inc)
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    Danaher Inc rabbit anti map2
    Fig. 3 | Role of the UPR and Wnt/β-catenin signaling in nuclear REST induction. a Immunolabeling of UPR activation (marker BiP/GRP78, red), REST (green), β- catenin (magenta) and DNA (DAPI, blue) in 11-month-old 3xTg and WT mice shows coordinate upregulation of BiP, nuclear REST and nuclear β-catenin expression in 3xTg mice. b Correlation between nuclear β-catenin and nuclear REST (left graph), BiP and nuclear REST (middle graph) and BiP and nuclear β-catenin (right graph) levels in the hippocampus CA1 region of 11-month-old 3xTg mice. Shown are the mean fluorescence intensity values for nuclear REST in individual CA1 neurons from n = 3 3xTg mice. a.u.- arbitrary units. The Pearson correlation coefficient (r) and P- value are shown. c Immunolabeling of REST (green) and neuron marker <t>MAP2</t> (red) in 3xTg primary cortical neurons (PCNs) shows nuclear REST in neurons treated with vehicle and decreased nuclear REST in 3xTg neurons after a 24-h treatment with the Wnt/β-catenin inhibitors Dickkopf 1 (DKK1), XAV939 and ICG001, or the PERK inhibitor GSK2606414. d Quantification of average nuclear REST levels in WT PCNs, as well as 3xTg PCNs treated with vehicle or the individual drugs. The P-
    Rabbit Anti Map2, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Journal: Cell reports

    Article Title: Opposing roles of p38α-mediated phosphorylation and PRMT1-mediated arginine methylation in driving TDP-43 proteinopathy

    doi: 10.1016/j.celrep.2024.115205

    Figure Lengend Snippet:

    Article Snippet: Goat Anti-MAP2 , Phosphosolutions , Cat # 1099;RRID:AB_2752241.

    Techniques: Virus, Recombinant, Protease Inhibitor, Membrane, Western Blot, Stripping, Modification, Transfection, Fractionation, Cell Culture, Lysis, Magnetic Beads, Electron Microscopy, Staining, Bicinchoninic Acid Protein Assay, Kinase Assay, LDH Cytotoxicity Assay, Mutagenesis, CyQUANT Assay, Sequencing, Negative Control, DNA Sequencing, Plasmid Preparation, Software, Imaging

    SMN expression alleviates reduced snRNP biogenesis in GEMIN5-mediated disease. a Confocal images of control and mutant GEMIN5 L1068P neurons transduced with EGFP and lentiviral EGFP-SMN from the same set of neuronal differentiations. The neurons were probed for the snRNP marker Y12 (Sm), GFP, and the neuronal marker microtubule-associated protein 2 (MAP2). The cell nuclei were stained with DAPI. Scale bar = 10 μm. b Quantitative analysis observing a significant decrease in nuclear snRNP expression in L1068P neurons after lentiviral transduction of EGFP. Lentiviral transduction of EGFP-SMN significantly rescues the levels of nuclear snRNP expression in L1068P neurons (n = 75–100 neurons, one-way ANOVA w/ Tukey’s multiple comparisons). Error bars indicate S.E.M. *p < 0.05; **p < 0.01; ****p < 0.0001. NS, not significant. c Confocal images of control and mutant GEMIN5 H913R neurons transduced with EGFP and lentiviral EGFP-SMN from the same set of neuronal differentiations. The neurons were probed for the small nuclear ribonucleoprotein interacting partner, Coilin (cajal bodies, CBs), GFP, and GEMIN5. The cell nuclei were stained with DAPI. Scale bar = 10 μm. d Quantitative analysis observing the average number of CBs per cell in control and mutant H913R neuronal cells with EGFP and EGFP-SMN lentiviral expression. Only CBs above 0.2 microns were quantified (n = 60–80 neurons, one-way ANOVA w/ Tukey’s multiple comparisons). Error bars indicate S.E.M. **p < 0.01; NS, not significant. e The percentage of cells with CBs in control and mutant H913R neuronal cells with EGFP and EGFP-SMN expression (n = 60–80 neurons, one-way ANOVA w/ Bonferroni test). Error bars indicate S.E.M. **p < 0.01; NS, not significant. f Representative gel displaying complete in vitro snRNP assembly formation using 3′ Cy3-biotin-labeled U1 snRNA and the cytoplasmic extract from control and L1068P neurons with lentirviral SMN and ASO N1 administration. Untransfected HEK293T cells and HEK293T transfected with GEMIN5 shRNA were used as a positive control for assembly formation. g Representative quantification of 3 biological replicate sample displaying the intensity of the snRNP assembly between groups (n = 3, one-way ANOVA w/ Tukey’s multiple comparisons). Error bars indicate S.E.M. *p < 0.05; **p < 0.01. NS, not significant. h Confocal images of the ventral nerve cord (VNC) of control and ubiquitously expressing GEMIN5 KD Drosophila larvae with and without Smn over expression stained with the snRNP marker Sm (Y12) and nuclei (DAPI). Loss of GEMIN5 in vivo results in a reduction of nuclear Sm which is rescued by upregulation of Smn. Scale bar = 10 μM. i. Confocal images of the ventral nerve cord (VNC) of control and ubiquitously expressing GEMIN5 KD Drosophila larvae with and without Smn over expression stained with the small nuclear ribonucleoprotein interacting partner, dCoilin (CBs) and nuclei (DAPI). Loss of GEMIN5 in vivo results in a drastic reduction of CB formation which is rescued by upregulation of Smn. Scale bar = 10 μM

    Journal: Acta neuropathologica

    Article Title: SMN regulates GEMIN5 expression and acts as a modifier of GEMIN5-mediated neurodegeneration

    doi: 10.1007/s00401-023-02607-8

    Figure Lengend Snippet: SMN expression alleviates reduced snRNP biogenesis in GEMIN5-mediated disease. a Confocal images of control and mutant GEMIN5 L1068P neurons transduced with EGFP and lentiviral EGFP-SMN from the same set of neuronal differentiations. The neurons were probed for the snRNP marker Y12 (Sm), GFP, and the neuronal marker microtubule-associated protein 2 (MAP2). The cell nuclei were stained with DAPI. Scale bar = 10 μm. b Quantitative analysis observing a significant decrease in nuclear snRNP expression in L1068P neurons after lentiviral transduction of EGFP. Lentiviral transduction of EGFP-SMN significantly rescues the levels of nuclear snRNP expression in L1068P neurons (n = 75–100 neurons, one-way ANOVA w/ Tukey’s multiple comparisons). Error bars indicate S.E.M. *p < 0.05; **p < 0.01; ****p < 0.0001. NS, not significant. c Confocal images of control and mutant GEMIN5 H913R neurons transduced with EGFP and lentiviral EGFP-SMN from the same set of neuronal differentiations. The neurons were probed for the small nuclear ribonucleoprotein interacting partner, Coilin (cajal bodies, CBs), GFP, and GEMIN5. The cell nuclei were stained with DAPI. Scale bar = 10 μm. d Quantitative analysis observing the average number of CBs per cell in control and mutant H913R neuronal cells with EGFP and EGFP-SMN lentiviral expression. Only CBs above 0.2 microns were quantified (n = 60–80 neurons, one-way ANOVA w/ Tukey’s multiple comparisons). Error bars indicate S.E.M. **p < 0.01; NS, not significant. e The percentage of cells with CBs in control and mutant H913R neuronal cells with EGFP and EGFP-SMN expression (n = 60–80 neurons, one-way ANOVA w/ Bonferroni test). Error bars indicate S.E.M. **p < 0.01; NS, not significant. f Representative gel displaying complete in vitro snRNP assembly formation using 3′ Cy3-biotin-labeled U1 snRNA and the cytoplasmic extract from control and L1068P neurons with lentirviral SMN and ASO N1 administration. Untransfected HEK293T cells and HEK293T transfected with GEMIN5 shRNA were used as a positive control for assembly formation. g Representative quantification of 3 biological replicate sample displaying the intensity of the snRNP assembly between groups (n = 3, one-way ANOVA w/ Tukey’s multiple comparisons). Error bars indicate S.E.M. *p < 0.05; **p < 0.01. NS, not significant. h Confocal images of the ventral nerve cord (VNC) of control and ubiquitously expressing GEMIN5 KD Drosophila larvae with and without Smn over expression stained with the snRNP marker Sm (Y12) and nuclei (DAPI). Loss of GEMIN5 in vivo results in a reduction of nuclear Sm which is rescued by upregulation of Smn. Scale bar = 10 μM. i. Confocal images of the ventral nerve cord (VNC) of control and ubiquitously expressing GEMIN5 KD Drosophila larvae with and without Smn over expression stained with the small nuclear ribonucleoprotein interacting partner, dCoilin (CBs) and nuclei (DAPI). Loss of GEMIN5 in vivo results in a drastic reduction of CB formation which is rescued by upregulation of Smn. Scale bar = 10 μM

    Article Snippet: The HEK293T cells and neurons were fixed in 4% paraformaldehyde (PFA) for 15 min and blocked in 0.1% Triton-X in PBS and 5% normal goat serum for 1 h. The cells were treated overnight with the following antibodies: rabbit anti-GEMIN5 (Millipore Sigma HPA037393, 1:1,000), mouse anti-Smb (Y12) (NovusBio, 1:500), mouse anti-SMN (BD transduction 610,646, 1:1,000), mouse anti-coilin (Santa Cruz sc-55594, 1:500), chicken anti-beta-III Tubulin (NOVUS Biologicals NB100–1612-1:1,000), and goat anti-MAP2 (Synaptic System-188 004, 1:1,000).

    Techniques: Expressing, Control, Mutagenesis, Transduction, Marker, Staining, In Vitro, Labeling, Transfection, shRNA, Positive Control, Over Expression, In Vivo

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Non-canonical function of ADAM10 in presynaptic plasticity

    doi: 10.1007/s00018-024-05327-8

    Figure Lengend Snippet:

    Article Snippet: Anti-MAP2 (goat) , Santa Cruz , Cat#sc-5359; RRID:AB_649160.

    Techniques: Plasmid Preparation, Recombinant, Blocking Assay, Control, In Situ, Knock-Out, Software, Microscopy

    Fig. 3 | Role of the UPR and Wnt/β-catenin signaling in nuclear REST induction. a Immunolabeling of UPR activation (marker BiP/GRP78, red), REST (green), β- catenin (magenta) and DNA (DAPI, blue) in 11-month-old 3xTg and WT mice shows coordinate upregulation of BiP, nuclear REST and nuclear β-catenin expression in 3xTg mice. b Correlation between nuclear β-catenin and nuclear REST (left graph), BiP and nuclear REST (middle graph) and BiP and nuclear β-catenin (right graph) levels in the hippocampus CA1 region of 11-month-old 3xTg mice. Shown are the mean fluorescence intensity values for nuclear REST in individual CA1 neurons from n = 3 3xTg mice. a.u.- arbitrary units. The Pearson correlation coefficient (r) and P- value are shown. c Immunolabeling of REST (green) and neuron marker MAP2 (red) in 3xTg primary cortical neurons (PCNs) shows nuclear REST in neurons treated with vehicle and decreased nuclear REST in 3xTg neurons after a 24-h treatment with the Wnt/β-catenin inhibitors Dickkopf 1 (DKK1), XAV939 and ICG001, or the PERK inhibitor GSK2606414. d Quantification of average nuclear REST levels in WT PCNs, as well as 3xTg PCNs treated with vehicle or the individual drugs. The P-

    Journal: Nature communications

    Article Title: A neurodegeneration checkpoint mediated by REST protects against the onset of Alzheimer's disease.

    doi: 10.1038/s41467-023-42704-6

    Figure Lengend Snippet: Fig. 3 | Role of the UPR and Wnt/β-catenin signaling in nuclear REST induction. a Immunolabeling of UPR activation (marker BiP/GRP78, red), REST (green), β- catenin (magenta) and DNA (DAPI, blue) in 11-month-old 3xTg and WT mice shows coordinate upregulation of BiP, nuclear REST and nuclear β-catenin expression in 3xTg mice. b Correlation between nuclear β-catenin and nuclear REST (left graph), BiP and nuclear REST (middle graph) and BiP and nuclear β-catenin (right graph) levels in the hippocampus CA1 region of 11-month-old 3xTg mice. Shown are the mean fluorescence intensity values for nuclear REST in individual CA1 neurons from n = 3 3xTg mice. a.u.- arbitrary units. The Pearson correlation coefficient (r) and P- value are shown. c Immunolabeling of REST (green) and neuron marker MAP2 (red) in 3xTg primary cortical neurons (PCNs) shows nuclear REST in neurons treated with vehicle and decreased nuclear REST in 3xTg neurons after a 24-h treatment with the Wnt/β-catenin inhibitors Dickkopf 1 (DKK1), XAV939 and ICG001, or the PERK inhibitor GSK2606414. d Quantification of average nuclear REST levels in WT PCNs, as well as 3xTg PCNs treated with vehicle or the individual drugs. The P-

    Article Snippet: Additional primary antibodies were as follows: anti-human Aβ rabbit monoclonal IgG antibody (Cell Signaling, Cat. No. 8243); antihuman APP mouse monoclonal IgG antibody (clone 6E10; Covance, Catalog No. SIG-39320); anti-actin mouse monoclonal IgG antibody (clone ACTN05 (C4); ThermoFisher Scientific, CatalogNo.MA5-11869); anti-NeuN mouse monoclonal IgG antibody (clone A60, Millipore, MAB377); anti-MAP2 goat polyclonal IgG antibody (PhosphoSolutions, Catalog. No. 1099-MAP2); anti-CDK5 mouse monoclonal IgG antibody (clone 4E4; Novus Bio, Catalog No. NBP2-37602); anti-GSK3β mouse monoclonal IgG antibody (clone D5C5Z; Novus Bio, catalog No. NBP147470S); anti-PS1 C-terminal (CTF) rabbit monoclonal IgG antibody (clone EP2000Y; Abcam, Catalog No. ab76083); anti-PS1 N-terminal (NTF) rabbit polyclonal IgG antibody (231-f; made in the Yankner lab); anti-Nicastrin mouse monoclonal IgG antibody (clone 9C3; Biolegend, Catalog No. 852301); anti-Nicastrin rabbit polyclonal IgG antibody (Sigma Millipore, Catalog No. N1660); anti-PEN2 rabbit polyclonal IgG antibody (ProScience, Catalog No.3981); anti-PEN2 rabbit monoclonal IgG antibody (clone EPR9200; Abcam, Catalog No. ab154830); antiPEN2 rabbit polyclonal IgG (ProScience, Catalog No. 3981); antiTransferrin receptor mouse monoclonal IgG antibody (clone H68.4; ThermoFisher Scientific, Catalog No. 13-6800); anti-BiP/GRP78 mouse monoclonal IgG (clone C38; ThermoFisher Scientific, clone C38, CatalogNo.

    Techniques: Immunolabeling, Activation Assay, Marker, Expressing

    Fig. 5 | REST suppresses the tau kinases CDK5 and GSK3β. a, b Loss of REST in excitatory neurons increases CDK5 expression in cortex and hippocampus. aImmunolabelingforCDK5(green)andthe neuronalmarkerMAP2(magenta)in CA1 neurons of the hippocampus in 9-month-old 3xTg and 3xTg;cKO mice. b Quantification of CDK5 immunofluorescence intensity in the hippocampus and cortex of 9-month-old 3xTg (n = 4) and 3xTg;cKO (n = 4) mice. c, d Loss of a single REST allele increases CDK5 expression. c Immunolabeling for CDK5 (green) and MAP2 (magenta) in 29-month-old 3xTg and 3xTg;GT (heterozygous REST null) mice. d Quantification of CDK5 immunofluorescence intensity in 28–29-month-old 3xTg (n = 6) and 3xTg;GT (n = 6) mice. e, f Loss of REST in excitatory neurons increases GSKβ expression in cortex and hippocampus. e Immunolabeling for GSK3β (green) and MAP2 (magenta) in hippocampal CA1 neurons in 9-month-old 3xTg and 3xTg;cKO mice. f Quantification of GSK3β immunofluorescenceintensityin9-month-old3xTg(n = 4)and3xTg;cKO (n = 4) mice. g, h Loss of a single REST allele increases GSK3β expression.

    Journal: Nature communications

    Article Title: A neurodegeneration checkpoint mediated by REST protects against the onset of Alzheimer's disease.

    doi: 10.1038/s41467-023-42704-6

    Figure Lengend Snippet: Fig. 5 | REST suppresses the tau kinases CDK5 and GSK3β. a, b Loss of REST in excitatory neurons increases CDK5 expression in cortex and hippocampus. aImmunolabelingforCDK5(green)andthe neuronalmarkerMAP2(magenta)in CA1 neurons of the hippocampus in 9-month-old 3xTg and 3xTg;cKO mice. b Quantification of CDK5 immunofluorescence intensity in the hippocampus and cortex of 9-month-old 3xTg (n = 4) and 3xTg;cKO (n = 4) mice. c, d Loss of a single REST allele increases CDK5 expression. c Immunolabeling for CDK5 (green) and MAP2 (magenta) in 29-month-old 3xTg and 3xTg;GT (heterozygous REST null) mice. d Quantification of CDK5 immunofluorescence intensity in 28–29-month-old 3xTg (n = 6) and 3xTg;GT (n = 6) mice. e, f Loss of REST in excitatory neurons increases GSKβ expression in cortex and hippocampus. e Immunolabeling for GSK3β (green) and MAP2 (magenta) in hippocampal CA1 neurons in 9-month-old 3xTg and 3xTg;cKO mice. f Quantification of GSK3β immunofluorescenceintensityin9-month-old3xTg(n = 4)and3xTg;cKO (n = 4) mice. g, h Loss of a single REST allele increases GSK3β expression.

    Article Snippet: Additional primary antibodies were as follows: anti-human Aβ rabbit monoclonal IgG antibody (Cell Signaling, Cat. No. 8243); antihuman APP mouse monoclonal IgG antibody (clone 6E10; Covance, Catalog No. SIG-39320); anti-actin mouse monoclonal IgG antibody (clone ACTN05 (C4); ThermoFisher Scientific, CatalogNo.MA5-11869); anti-NeuN mouse monoclonal IgG antibody (clone A60, Millipore, MAB377); anti-MAP2 goat polyclonal IgG antibody (PhosphoSolutions, Catalog. No. 1099-MAP2); anti-CDK5 mouse monoclonal IgG antibody (clone 4E4; Novus Bio, Catalog No. NBP2-37602); anti-GSK3β mouse monoclonal IgG antibody (clone D5C5Z; Novus Bio, catalog No. NBP147470S); anti-PS1 C-terminal (CTF) rabbit monoclonal IgG antibody (clone EP2000Y; Abcam, Catalog No. ab76083); anti-PS1 N-terminal (NTF) rabbit polyclonal IgG antibody (231-f; made in the Yankner lab); anti-Nicastrin mouse monoclonal IgG antibody (clone 9C3; Biolegend, Catalog No. 852301); anti-Nicastrin rabbit polyclonal IgG antibody (Sigma Millipore, Catalog No. N1660); anti-PEN2 rabbit polyclonal IgG antibody (ProScience, Catalog No.3981); anti-PEN2 rabbit monoclonal IgG antibody (clone EPR9200; Abcam, Catalog No. ab154830); antiPEN2 rabbit polyclonal IgG (ProScience, Catalog No. 3981); antiTransferrin receptor mouse monoclonal IgG antibody (clone H68.4; ThermoFisher Scientific, Catalog No. 13-6800); anti-BiP/GRP78 mouse monoclonal IgG (clone C38; ThermoFisher Scientific, clone C38, CatalogNo.

    Techniques: Expressing, Immunolabeling